{"id":6652,"date":"2022-03-23T21:06:36","date_gmt":"2022-03-23T12:06:36","guid":{"rendered":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/?p=6652"},"modified":"2022-03-31T20:43:13","modified_gmt":"2022-03-31T11:43:13","slug":"clinical-application","status":"publish","type":"post","link":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/general\/clinical-application.html","title":{"rendered":"Clinical Application"},"content":{"rendered":"<h1>FRET\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306e\u81e8\u5e8a\u5fdc\u7528<br \/>\n\u301c\u5149\u8a3a\u65ad\u85ac\u306e\u958b\u767a\u3068\u4e8b\u696d\u5316\u301c<\/h1>\n<p>\u86cd\u5149\u30d0\u30a4\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306f\u3001\u751f\u304d\u305f\u7d30\u80de\u5185\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u5909\u5316\u3092\u3001\u9ad8\u611f\u5ea6\u304b\u3064\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u6280\u8853\u3067\u3059\u3002\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u3053\u306e\u7279\u9577\u3092\u6d3b\u304b\u3057\u3066\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3092\u81e8\u5e8a\u691c\u67fb\u306b\u5fdc\u7528\u3059\u308b\u3053\u3068\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u6162\u6027\u9aa8\u9ac4\u6027\u767d\u8840\u75c5\uff08chronic myeloid leukemia, CML\uff09\u306f\u3001\u9aa8\u9ac4\u306e\u9020\u8840\u5e79\u7d30\u80de\u306b\u7570\u5e38\u67d3\u8272\u4f53\uff08\u30d5\u30a3\u30e9\u30c7\u30eb\u30d5\u30a3\u30a2\u67d3\u8272\u4f53, Ph1\uff09\u304c\u5f62\u6210\u3055\u308c\u3001\u767d\u8840\u75c5\u306e\u539f\u56e0\u30bf\u30f3\u30d1\u30af\u8ceaBCR-ABL\u304c\u7d30\u80de\u5185\u306b\u51fa\u73fe\u3057\u767a\u75c7\u3059\u308b\u8840\u6db2\u306e\u304c\u3093\u3067\u3059\u3002BCR\uff70ABL\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u306b\u5bfe\u3059\u308b\uff13\u7a2e\u985e\u306e\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u304c\u6a19\u6e96\u6cbb\u7642\u306b\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u3057\u304b\u3057\u3001\u60a3\u8005\u3055\u3093\u3054\u3068\u306b\u3069\u306e\u85ac\u304c\u6709\u52b9\u306a\u306e\u304b\u3001\u5b9f\u969b\u306b\u6cbb\u7642\u3092\u59cb\u3081\u3066\u307f\u308b\u307e\u3067\u306f\u5206\u304b\u3089\u306a\u3044\u3053\u3068\u304c\u8ab2\u984c\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u6cbb\u7642\u524d\u304b\u3089\u500b\u3005\u306e\u60a3\u8005\u3055\u3093\u306b\u5bfe\u3057\u3066\u85ac\u306e\u6709\u52b9\u6027\u3092\u5224\u5b9a\u3057\u305f\u308a\u3001\u6cbb\u7642\u4e2d\u306b\u85ac\u5264\u8010\u6027\u7d30\u80de\u306e\u6709\u7121\u3092\u691c\u51fa\u3057\u305f\u308a\u3059\u308b\u6280\u8853\u304c\u3042\u308c\u3070\u3001\u306f\u3058\u3081\u304b\u3089\u52b9\u679c\u7684\u306a\u6cbb\u7642\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><a href=\"..\/wp-content\/uploads\/2014\/03\/bio_censor01.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-340\" src=\"..\/wp-content\/uploads\/2014\/03\/bio_censor01.jpg\" alt=\"bio_sensor01\" width=\"209\" height=\"161\" \/><\/a><\/p>\n<p><strong>\u56f31\u3000BCR-ABL\u6d3b\u6027\u6e2c\u5b9a\u30bb\u30f3\u30b5\u30fcPickles<\/strong><br \/>\nBCR-ABL\u306e\u57fa\u8cea\u3067\u3042\u308bCrkL\u306f\u30ea\u30f3\u9178\u5316\u3055\u308c\u308b\u3068\u69cb\u9020\u5909\u5316\u3059\u308b\u3002CrkL\u306e\u4e21\u7aef\u306bYFP\uff08Yellow fluorescent protein, \u9ec4\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3068CFP\uff08Cyan fluorescent protein, \u9752\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3092\u4ed8\u3051\u308b\u3053\u3068\u3067\u3001BCR-ABL\u306e\u6d3b\u6027\u306b\u5fdc\u7b54\u3057\u3066FRET\u304c\u751f\u3058\u308b\u30bb\u30f3\u30b5\u30fc\u306e\u958b\u767a\u306b\u6210\u529f\u3057\u305f\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306fBCR-ABL\u306e\u57fa\u8ceaCrkL\u3068FRET\u306e\u539f\u7406\u3092\u5229\u7528\u3057\u305f\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u3000Pickles\uff08Phosphorylation indicator of CrkL en substrate\u3001\u56f31\uff09\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u305d\u308c\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u5b9f\u969b\u306b\u60a3\u8005\u3055\u3093\u304b\u3089\u63a1\u53d6\u3057\u305fCML\u7d30\u80de\u304c\u3069\u306e\u3088\u3046\u306a\u85ac\u5264\u53cd\u5fdc\u6027\u3092\u793a\u3059\u304b\u3092\u4e00\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u89e3\u6790\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3057\u307e\u3057\u305f\uff08\u56f32\uff09\u3002<\/p>\n<p><a href=\"..\/wp-content\/uploads\/2014\/03\/bio_censor02.jpg\"><img loading=\"lazy\" src=\"..\/wp-content\/uploads\/2014\/03\/bio_censor02.jpg\" alt=\"bio_sensor02\" width=\"209\" height=\"157\" \/><\/a><\/p>\n<p><strong>\u56f32\u3000Pickles\u306b\u3088\u308b\u85ac\u52b9\u8a55\u4fa1\u30d7\u30ed\u30bb\u30b9<\/strong><br \/>\n\u60a3\u8005\u3055\u307e\u304b\u3089\u5f97\u305f\u8840\u7403\u304b\u3089\u3001\u5358\u6838\u7403\u5206\u753b\u3092\u5358\u96e2\u3057\u3001Pickles\u3092\u5c0e\u5165\u3059\u308b\u300224\u6642\u9593\u5f8c\u306b\u306f\u30bb\u30f3\u30b5\u30fc\u5206\u5b50\u306e\u86cd\u5149\u304c\u89b3\u5bdf\u53ef\u80fd\u3067\u3042\u308a\uff0c\u9855\u5fae\u93e1\u3067\u4e00\u3064\u4e00\u3064\u306eFRET\u52b9\u7387\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3002\u85ac\u5264\u51e6\u7406\u5f8c\u306b\u3059\u3079\u3066\u306e\u7d30\u80de\u3067BCR-ABL\u306e\u6d3b\u6027\u304c\u6291\u5236\uff08FRET\u4f4e\uff09\u3055\u308c\u3066\u3044\u308c\u3070\u85ac\u5264\u304c\u6709\u52b9\u3001\u62b5\u6297\u6027\u306e\u5834\u5408\u306b\u306fFRET\u306e\u9ad8\u3044\u7d30\u80de\u304c\u6b8b\u5b58\u3059\u308b\u3002<\/p>\n<p>\u672c\u6280\u8853\u3092\u6cbb\u7642\u524d\u306eCML\u60a3\u8005\u3055\u3093\u306b\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u6709\u52b9\u306a\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u3092\u5b9f\u969b\u306b\u4e88\u6e2c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u500b\u3005\u306e\u60a3\u8005\u3055\u3093\u306b\u30d5\u30a3\u30c3\u30c8\u3057\u305f\u6cbb\u7642\u85ac\u3092\u3042\u3089\u304b\u3058\u3081\u9078\u629e\u3067\u304d\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u672c\u624b\u6cd5\u306b\u306f\u300c\u7d30\u80de\u3092\u751f\u304d\u305f\u307e\u307e\u89e3\u6790\u3059\u308b\u300d\u3068\u3044\u3046\u3001\u3053\u308c\u307e\u3067\u306e\u691c\u67fb\u306b\u306f\u306a\u3044\u7279\u9577\u304c\u3042\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u85ac\u5264\u8010\u6027\u7d30\u80de\u306e\u307f\u3092\u96c6\u3081\u308b\u3053\u3068\u3082\u53ef\u80fd\u306a\u3053\u3068\u304b\u3089\u3001\u85ac\u5264\u8010\u6027\u7372\u5f97\u6a5f\u69cb\u306e\u89e3\u660e\u3078\u767a\u5c55\u3055\u305b\u308b\u3053\u3068\u3082\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<br \/>\n\u3055\u3089\u306b\u3001\u4ed6\u306e\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u3092\u6e2c\u5b9a\u3059\u308b\u5149\u8a3a\u65ad\u85ac\u3092\u958b\u767a\u3059\u308b\u3053\u3068\u3067\u3001\u7406\u8ad6\u7684\u306b\u306f\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u3092\u7528\u3044\u308b\u3059\u3079\u3066\u306e\u304c\u3093\u306b\u5fdc\u7528\u62e1\u5927\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u672c\u7814\u7a76\u306f\u3001\u79d1\u5b66\u6280\u8853\u632f\u8208\u6a5f\u69cb\uff08JST\uff09\u306e\u300c\u793e\u4f1a\u9084\u5143\u52a0\u901f\u30d7\u30ed\u30b0\u30e9\u30e0\uff08SCORE\uff09\u300d\uff082019\u5e74\u5ea6\uff09\u3001\u30c6\u30eb\u30e2\u751f\u547d\u79d1\u5b66\u632f\u8208\u8ca1\u56e3\u306e\u300c\u7814\u7a76\u958b\u767a\u52a9\u6210\uff08\u958b\u767a\u52a9\u6210\uff09\u300d\u3001\u56fd\u7acb\u7814\u7a76\u958b\u767a\u6cd5\u4eba\u65b0\u30a8\u30cd\u30eb\u30ae\u30fc\u30fb\u7523\u696d\u6280\u8853\u7dcf\u5408\u958b\u767a\u6a5f\u69cb\uff08NEDO\uff09\u306e\u300c\u7814\u7a76\u958b\u767a\u578b\u30b9\u30bf\u30fc\u30c8\u30a2\u30c3\u30d7\u652f\u63f4\u4e8b\u696d\/NEDO Entrepreneurs Program(NEP)\u30bf\u30a4\u30d7B\u300d\uff082021\u5e74\u5ea6\uff09\u306e\u3054\u652f\u63f4\u3092\u3044\u305f\u3060\u304d\u3001\u4e00\u65e5\u3082\u65e9\u304f\u60a3\u8005\u3055\u307e\u306e\u624b\u5143\u306b\u672c\u6280\u8853\u304c\u5c4a\u304f\u3088\u3046\u306b\u3001\u4e8b\u696d\u5316\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n\u8a73\u3057\u304f\u306f<a href=\"https:\/\/www.horizonillumination.co.jp\/\">HILO\u682a\u5f0f\u4f1a\u793e<\/a>\u306e\u30da\u30fc\u30b8\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u30fb\u53c2\u8003\u6587\u732e\u3011<\/strong><\/p>\n<ul>\n<li>A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, &amp; <strong><u>Ohba Y<\/u><\/strong>*. <em><strong>Clin. Cancer\u00a0Res.<\/strong><\/em> 16(15): 3964-3975, 2010<\/li>\n<\/ul>\n<p style=\"padding-left: 70px;\">Commentary on this paper: <em><strong>Clin. Cancer Res.<\/strong><\/em> 16(15): 3822-3824 (2010)<\/p>\n<ul>\n<li>Improved FRET biosensor for the measurement of BCR-ABL activity in chronic myeloid leukemia cells. Horiguchi M, Fujioka M, Kondo T, Fujioka Y, Li X, Horiuchi K, Satoh A, Nepal P, Nishide S, Nanbo A, Teshima T, and <strong><u>Ohba Y<\/u><\/strong>*.\u00a0<em><strong>Cell Struct Funct<\/strong><\/em> 42, 15-26, 2017<\/li>\n<li>Pre-treatment evaluation of FRET-based drug sensitivity test for patients with CML treated with dasatinib. Kondo T, et al, <strong><em>Cancer Sci. <\/em><\/strong>109(7): 2256-2265, 2018<\/li>\n<li>Clinical efficacy and safety of first-line nilotinib therapy and evaluation of the clinical utility of the FRET-based drug sensitivity test. Kondo T, et al. <strong><em>Int. J. Hematol.<\/em><\/strong> 110(4): 482-489, 2019<\/li>\n<li>\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7814\u7a762008-&#8217;09 \u2015\u75be\u60a3\u767a\u75c7\u306e\u5206\u5b50\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u5b9f\u73fe\u5316\u3059\u308b\u5206\u5b50\u6a19\u7684\u85ac\u958b\u767a\u2015\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u53ef\u8996\u5316\u3068\u305d\u306e\u5275\u85ac\u3078\u306e\u5fdc\u7528 \u2002 <strong><u>\u5927\u5834\u96c4\u4ecb<\/u><\/strong>\u3001\u6d25\u7530\u771f\u5bff\u7f8e \u2002 <strong>\u5b9f\u9a13\u533b\u5b66<\/strong> 25(15)\uff08\u5897\u520a\uff09\uff1a 206-213 (2506-2513), 2008<\/li>\n<li>\u30d0\u30a4\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306e\u57fa\u790e\u3068\u5fdc\u7528 \u2002 \u6d25\u7530\u771f\u5bff\u7f8e\u3001<strong> <u>\u5927\u5834\u96c4\u4ecb<\/u><\/strong> \u2002 <strong>\u7d30\u80de<\/strong> 41(11): 462-463, 2009<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\n<ul>\n<li><a href=\"http:\/\/aacrnews.wordpress.com\/2010\/07\/29\/new-lab-test-could-identify-imatinib-resistance\/\" target=\"_blank\" rel=\"noopener noreferrer\">AACR\uff08\u7c73\u56fd\u304c\u3093\u5b66\u4f1a\uff09<\/a><\/li>\n<li><a href=\"http:\/\/www.hokudai.ac.jp\/bureau\/topics\/press_release\/100729_pr_med.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">\u5317\u6d77\u9053\u5927\u5b66<\/a><\/li>\n<li>\u65e5\u7d4c\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\uff08\u30ea\u30f3\u30af\u5207\u308c\uff09<\/li>\n<\/ul>\n<\/li>\n<li>\u5831\u9053\n<ul>\n<li>\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30cb\u30e5\u30fc\u30b9\u5317\u6d77\u9053\uff08NHK\u30017\/29)<\/li>\n<li>\u9053\u65b0\u30cb\u30e5\u30fc\u30b9\uff08\u30c6\u30ec\u30d3\u5317\u6d77\u9053\u30017\/31\uff09<\/li>\n<li>\u5317\u6d77\u9053\u65b0\u805e\uff087\/30\u671d\u520a\uff09<\/li>\n<li>\u8aad\u58f2\u65b0\u805e\uff087\/29\u671d\u520a\uff09<\/li>\n<li>\u6bce\u65e5\u65b0\u805e\uff087\/30\u671d\u520a\uff09<\/li>\n<li>\u30ed\u30a4\u30bf\u30fc\u901a\u4fe1<\/li>\n<li>BTJ \u30a2\u30ab\u30c7\u30df\u30c3\u30af 7\/29\u306e\u30a2\u30af\u30bb\u30b9\u30e9\u30f3\u30ad\u30f3\u30b0\uff11\u4f4d\uff01<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>\u7d30\u80de\u751f\u7406\u306e\u4ed6\u306e\u7814\u7a76\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/research.html\">\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>FRET\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306e\u81e8\u5e8a\u5fdc\u7528 \u301c\u5149\u8a3a &hellip; <\/p>\n","protected":false},"author":2,"featured_media":62,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[8],"tags":[],"_links":{"self":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/posts\/6652"}],"collection":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/comments?post=6652"}],"version-history":[{"count":4,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/posts\/6652\/revisions"}],"predecessor-version":[{"id":6965,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/posts\/6652\/revisions\/6965"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/media\/62"}],"wp:attachment":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/media?parent=6652"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/categories?post=6652"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/tags?post=6652"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}