{"id":6983,"date":"2022-04-11T11:24:14","date_gmt":"2022-04-11T02:24:14","guid":{"rendered":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/?page_id=6983"},"modified":"2022-12-20T15:43:10","modified_gmt":"2022-12-20T06:43:10","slug":"research","status":"publish","type":"page","link":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/research.html","title":{"rendered":"\u7814\u7a76"},"content":{"rendered":"<h1>\u30d3\u30b8\u30e7\u30f3<\/h1>\n<h2>\u751f\u547d\uff08\u3044\u306e\u3061\uff09\u306e\u7406\uff08\u3053\u3068\u308f\u308a\uff09\u306e\u5168\u8c8c\u89e3\u660e<\/h2>\n<h3>\u301c\u57fa\u672c\u5358\u4f4d\u300c\u7d30\u80de\u300d\u306e\u52d5\u4f5c\u539f\u7406\u306e\u69cb\u6210\u7684\u7406\u89e3\u3092\u901a\u3058\u3066\u301c<\/h3>\n<p>60\u5146\u500b\u3068\u3082\u8a00\u308f\u308c\u308b\u30d2\u30c8\u306e\u7d30\u80de\u306f\u3001\u6c7a\u3057\u3066\u5e38\u306b\u540c\u3058\u72b6\u614b\u306b\u3042\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u500b\u3005\u306e\u7d30\u80de\u306f\u305f\u3048\u305a\u5916\u754c\u306e\u74b0\u5883\u5909\u5316\u306b\u5fdc\u7b54\u3057\u3066\u304a\u308a\u3001\u305d\u308c\u3089\u304c\u4e92\u3044\u306b\u5354\u8abf\u3057\u3066\u751f\u7406\u6a5f\u80fd\u3092\u767a\u63ee\u3059\u308b\u3053\u3068\u3067\u3001\uff11\u500b\u306e\u751f\u4f53\u3068\u3057\u3066\u306e\u6052\u5e38\u6027\u304c\u4fdd\u305f\u308c\u3066\u3044\u307e\u3059\uff08\u52d5\u7684\u6052\u5e38\u6027\u306e\u7dad\u6301\uff09\u3002\u305d\u306e\u4ed5\u7d44\u307f\u3092\u3001\u5c11\u3057\u304f\u308f\u3057\u304f\u304a\u8a71\u3057\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u7d30\u80de\u306f\u3001\u305d\u306e\u3072\u3068\u3064\u3072\u3068\u3064\u304c\u3001\u8102\u8cea\u4e8c\u91cd\u5c64\u306b\u3055\u307e\u3056\u307e\u306a\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u57cb\u3081\u3089\u308c\u305f\u300c\u7d30\u80de\u819c\u300d\u3067\u56f2\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u6e29\u5ea6\u5909\u5316\u3084\u5916\u6765\u7269\u8cea\u306e\u63a5\u89e6\u306a\u3069\u3001\u5916\u90e8\u304b\u3089\u306e\u523a\u6fc0\u3092\u5bdf\u77e5\u3059\u308b\u3068\u3001\u6700\u521d\u306b\u7d30\u80de\u819c\u304c\u30ca\u30ce\u30ec\u30d9\u30eb\uff081\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\u306f1\u30df\u30ea\u30e1\u30fc\u30c8\u30eb\u306e1\/1,000,000\u306e\u30b5\u30a4\u30ba\uff09\u306e\u5909\u5316\u3092\u8d77\u3053\u3057\u307e\u3059\u3002\u3053\u308c\u3092\u79c1\u305f\u3061\u306f\u300c\u7d30\u80de\u819c\u30ca\u30ce\u52d5\u614b\u300d\u3068\u547c\u3093\u3067\u3044\u307e\u3059\u3002\u305d\u3057\u3066\u3001\u305d\u308c\u304c\u5f15\u304d\u91d1\u3068\u306a\u3063\u3066\u3001\u7d30\u80de\u5185\u3067\u306f\u523a\u6fc0\u306b\u5fdc\u3058\u305f\u4e00\u9023\u306e\u5206\u5b50\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\u3053\u308c\u304c\u300c\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u300d\u3067\u3059\u3002<\/p>\n<p>\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u304c\u7cbe\u78ba\u306b\u5236\u5fa1\u3055\u308c\u308b\u3053\u3068\u304c\u3001\u7d30\u80de\u3001\u3072\u3044\u3066\u306f\u751f\u4f53\u304c\u6b63\u3057\u3044\u751f\u7406\u6a5f\u80fd\u3092\u767a\u73fe\u3059\u308b\u3046\u3048\u3067\u91cd\u8981\u3067\u3059\u3002\u3082\u3063\u3068\u7aef\u7684\u306b\u3044\u3048\u3070\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306f\u751f\u547d\u73fe\u8c61\u306e\u6839\u5e79\u3092\u306a\u3059\u3001\u3068\u3082\u8868\u73fe\u3067\u304d\u308b\u3067\u3057\u3087\u3046\u3002<\/p>\n<p>\u3055\u3089\u306b\u3001\u7d30\u80de\u306e\u4e2d\u306b\u306f\u3001\u305d\u308c\u305e\u308c\u4e00\u5b9a\u306e\u6a5f\u80fd\u3092\u3082\u3063\u305f\u300c\u30aa\u30eb\u30ac\u30cd\u30e9\uff08\u5c0f\u5668\u5b98\uff09\u300d\u304c\u5b58\u5728\u3057\u3001\u30aa\u30eb\u30ac\u30cd\u30e9\u3082\u307e\u305f\u819c\u3067\u56f2\u307e\u308c\u305f\u69cb\u9020\u3092\u3057\u3066\u3044\u307e\u3059\u3002\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u3088\u3063\u3066\u3001\u30aa\u30eb\u30ac\u30cd\u30e9\u306e\u819c\u30ca\u30ce\u52d5\u614b\u3082\u5909\u5316\u3057\u3001\u3055\u3089\u306a\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u884c\u3046\u3053\u3068\u3092\u79c1\u305f\u3061\u306f\u898b\u51fa\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u7d30\u80de\u819c\u3068\u30aa\u30eb\u30ac\u30cd\u30e9\u3001\u4e21\u65b9\u306e\u300c\u819c\u30ca\u30ce\u52d5\u614b\u300d\u306b\u3064\u3044\u3066\u306f\u3001\u307e\u3060\u307e\u3060\u5206\u304b\u3063\u3066\u3044\u306a\u3044\u3053\u3068\u304c\u305f\u304f\u3055\u3093\u3042\u308a\u307e\u3059\u3002\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3082\u540c\u69d8\u3067\u3059\u3002 \u79c1\u305f\u3061\u306f\u3001\u3053\u308c\u3089\u306e\u5236\u5fa1\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u300c\u30d0\u30a4\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u6280\u8853\u300d\u3092\u6b66\u5668\u306b\u53ef\u8996\u5316\u3001\u89b3\u5bdf\u3057\u3001\u5f97\u3089\u308c\u305f\u7d50\u679c\u3092\u8003\u7a76\u3057\u307e\u3059\u3002\u305d\u3053\u304b\u3089\u3001\u751f\u547d\u306e\u3057\u304f\u307f\u306b\u3064\u3044\u3066\u5305\u62ec\u7684\u306a\u7406\u89e3\u3092\u6df1\u3081\u307e\u3059\u3002<\/p>\n<h3>\u3055\u3042\u3001\u898b\u3066\u307f\u3088\u3046\u3001\u751f\u547d\u306e\u55b6\u307f\u3092\u3002\u5408\u8a00\u8449\u306f\u201dSeeing is believing\u201d\u3067\u3059\u3002<\/h3>\n<p>&nbsp;<\/p>\n<p id=\"recruit\">\n<h2>\u4eba\u6750\u80b2\u6210\u65b9\u91dd<\/h2>\n<h3>\u77e5\u306e\u5275\u9020\u3068\u305d\u308c\u3092\u62c5\u3046\u4eba\u6750\u80b2\u6210\u306e\u5834\u306e\u63d0\u4f9b<\/h3>\n<p>\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u304b\u3089\u3001\u6559\u79d1\u66f8\u306e\uff11\u30da\u30fc\u30b8\u304c\u66f8\u304d\u63db\u3048\u308b\u3088\u3046\u306a\u6210\u679c\u304c\u767a\u4fe1\u3067\u304d\u308c\u3070\u3001\u3053\u308c\u307b\u3069\u5b09\u3057\u3044\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3068\u306f\u3044\u3048\u3001\u81ea\u5206\u305f\u3061\u3060\u3051\u3067\u3067\u304d\u308b\u3053\u3068\u306f\u305d\u308c\u307b\u3069\u591a\u304f\u306f\u306a\u3044\u306e\u3082\u73fe\u5b9f\u3067\u3001\u5f8c\u9032\u306e\u79d1\u5b66\u8005\u3092\u80b2\u3066\u308b\u3053\u3068\u306f\u5927\u4e8b\u306a\u8cac\u52d9\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u7814\u7a76\u5ba4\u306e\u30e1\u30f3\u30d0\u30fc\u3072\u3068\u308a\u3072\u3068\u308a\u306e\u6210\u9577\u306e\u5b9f\u611f\u3068\u5922\u3092\u5b9f\u73fe\u3059\u308b\u5834\u3092\u3001\u7814\u7a76\u6d3b\u52d5\u3092\u901a\u3058\u3066\u5f62\u6210\u3057\u305f\u3044\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u3001\u73fe\u5728\u3084\u305d\u306e\u5f8c\u306e\u30ad\u30e3\u30ea\u30a2\u306b\u304a\u3044\u3066\u3001\u30d7\u30ed\u30d5\u30a7\u30c3\u30b7\u30e7\u30ca\u30eb\u304b\u3064\u30b0\u30ed\u30fc\u30d0\u30eb\u306a\u4eba\u7269\u305f\u308b\u4eba\u6750\u306e\u80b2\u6210\u306b\u8ca2\u732e\u3057\u305f\u3044\u3068\u601d\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u7814\u7a76\u5ba4\u3078\u306e\u53c2\u52a0\uff08\u5e0c\u671b\uff09\u8005\u306b\u306f\u3001\u300c\u81ea\u5206\u304c\u5b66\u3073\u305f\u3044\u3053\u3068\u3084\u6c42\u3081\u305f\u3044\u3082\u306e\u300d\u306b\u52a0\u3048\u3066\u3001\u300c\u81ea\u5206\u304c\u7814\u7a76\u5ba4\u306b\u8ca2\u732e\u3067\u304d\u308b\u3082\u306e\u300d\u306e\u4e21\u65b9\u3092\u63a2\u3057\u3066\u6b32\u3057\u3044\u3067\u3059\u3002\u7814\u7a76\u5ba4\u306b\u5165\u3063\u3066\u81ea\u5206\u81ea\u8eab\u306b\u5f97\u308b\u3082\u306e\u304c\u306a\u3051\u308c\u3070\u610f\u5473\u304c\u7121\u3044\u306e\u306f\u3082\u3061\u308d\u3093\u3067\u3059\u304c\u3001\u9006\u306b\u300c\u5927\u5b66\u3060\u304b\u3089\u5b66\u3076\u5834\u6240\u300d\u3068\u3057\u304b\u8003\u3048\u3066\u3044\u306a\u3044\u4eba\u306b\u306f\u3001\u7814\u7a76\u8005\u3068\u3057\u3066\uff08\u3042\u308b\u3044\u306f\u305d\u306e\u5f8c\u4ed6\u306e\u8077\u7a2e\u306b\u3064\u3044\u3066\u3082\uff09\u5927\u304d\u306a\u6210\u9577\u306f\u898b\u8fbc\u3081\u306a\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u4e21\u65b9\u3092\u898b\u3064\u3051\u305d\u308c\u3089\u3092\u6975\u3081\u305f\u4eba\u304c\u3044\u3044\u4ed5\u4e8b\u3092\u3057\u3001\u81ea\u5206\u81ea\u8eab\u3082\u6210\u9577\u3092\u5b9f\u611f\u3057\u306a\u304c\u3089\u80b2\u3063\u3066\u3044\u3063\u3066\u304f\u308c\u308b\u3082\u306e\u3060\u3068\u78ba\u4fe1\u3057\u3066\u3044\u307e\u3059\u3057\u3001\u305d\u306e\u3088\u3046\u306a\u5834\u3092\u63d0\u4f9b\u3057\u305f\u3044\u3068\u601d\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h1>\u7528\u8a9e\u96c6<\/h1>\n<p>\u5f53\u7814\u7a76\u5ba4\u306e\u7814\u7a76\u3092\u7406\u89e3\u3059\u308b\u52a9\u3051\u306b\u306a\u308b\u3001\u3042\u308b\u3044\u306f\u3088\u304f\u4f7f\u308f\u308c\u308b\u5358\u8a9e\u306e\u3068\u305d\u306e\u89e3\u8aac\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054<\/h2>\n<p>\u7d30\u80de\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306f\u4e00\u822c\u306b\u3001\u7d30\u80de\u8868\u9762\u306e\u53d7\u5bb9\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u30ea\u30ac\u30f3\u30c9\u7269\u8cea\u304c\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u5f15\u304d\u8d77\u3053\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u3092\u5f15\u304d\u91d1\u306b\u3001\u7d30\u80de\u5185\u3067\u9023\u7d9a\u7684\u306b\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u304c\u8d77\u3053\u308a\u3001\u30b7\u30b0\u30ca\u30eb\u304c\u79e9\u5e8f\u6b63\u3057\u304f\u5897\u5e45\u30fb\u4f1d\u9054\u3055\u308c\u3066\u3044\u304d\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u7d30\u80de\u5185\u5916\u306b\u5b58\u5728\u3059\u308b\u7279\u7570\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7ffb\u8a33\u5f8c\u4fee\u98fe\u3084\u69cb\u9020\u5909\u5316\u306a\u3069\u304c\u751f\u3058\u3001\u305d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u3084\u6a5f\u80fd\u304c\u5909\u5316\u3057\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u304c\u79e9\u5e8f\u6b63\u3057\u304f\u6642\u9593\u7684\u30fb\u7a7a\u9593\u7684\u306b\u53b3\u5bc6\u306b\u5236\u5fa1\u3055\u308c\u308b\u3053\u3068\u3067\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u3084\u7d30\u80de\u5f62\u614b\u306e\u5909\u5316\u306a\u3069\u306e\u8868\u73fe\u5f62\u304c\u898f\u5b9a\u3055\u308c\u3001\u6700\u7d42\u7684\u306b\u306f\u751f\u4f53\u53cd\u5fdc\u304c\u5236\u5fa1\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/p>\n<h2>\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea<\/h2>\n<p>\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08green fluorescent protein, GFP\uff09\u306f\u3001\u30aa\u30ef\u30f3\u30af\u30e9\u30b2\u306e\u767a\u5149\u5668\u5b98\u304b\u3089\u5358\u96e2\u3055\u308c\u305f\u300c\u5149\u308b\u300d\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u30021992\u5e74\u306b\u305d\u306ecDNA\u304c\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u3066\u4ee5\u6765\u3001\u5404\u7a2e\u7d30\u80de\u306b\u86cd\u5149\u7269\u8cea\u3092\u5c0e\u5165\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u7d30\u80de\u5185\u306e\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u52d5\u614b\u3084\u74b0\u5883\u5909\u5316\u306e\u53ef\u8996\u5316\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002 \u306a\u304a\u3001\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u898b\u3068\u5fdc\u7528\u306b\u3088\u3063\u3066\u3001\u4e0b\u6751\u8129\u535a\u58eb\u306f2008\u5e74\u3001\u65e5\u672c\u4eba\u3067\uff15\u4eba\u76ee\u3068\u306a\u308b\u30ce\u30fc\u30d9\u30eb\u5316\u5b66\u8cde\u3092\u53d7\u8cde\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h2>FRET<\/h2>\n<p>\u30d5\u30a7\u30eb\u30b9\u30bf\u30fc\u306e\u5171\u9cf4\u30a8\u30cd\u30eb\u30ae\u30fc\u79fb\u52d5\uff08F\u00f6rster resonance energy transfer, FRET\uff09\u306f\u3001\u4e8c\u3064\u306e\u86cd\u5149\u5206\u5b50\u9593\u3067\u30a8\u30cd\u30eb\u30ae\u30fc\u304c\u79fb\u52d5\u3059\u308b\u73fe\u8c61\u3067\u3059\u3002GFP\u306e\u5909\u7570\u4f53\u3092\u7528\u3044\u308b\u5834\u5408\u306b\u306f\u30b7\u30a2\u30f3\u8272\u306e\u5909\u7570\u4f53\uff08\u3042\u308b\u3044\u306f\u5358\u306b\u30b7\u30a2\u30f3\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceacyan fluorescent protein, CFP\uff09\u3068\u9ec4\u8272\u5909\u7570\u4f53\uff08YFP\uff09\u304c\u3088\u304f\u7528\u3044\u3089\u308c\u3001CFP\u3068YFP\u304c\u8fd1\u63a5\u3057\u305f\u5834\u5408\u306b\u306e\u307fCFP\u304b\u3089YFP\u3078\u306eFRET\u304c\u751f\u3058\u307e\u3059\uff08\u56f31\uff09\u3002\u751f\u304d\u305f\u7d30\u80de\u5185\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u5909\u5316\u3084\u6d3b\u6027\u5316\u3092\u3001\u9ad8\u611f\u5ea6\u304b\u3064\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p><a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/fret.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-345\" src=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/fret.jpg\" alt=\"\" width=\"200\" height=\"95\" \/><\/a><\/p>\n<p><strong>\u56f31\u3000FRET\u306e\u539f\u7406<\/strong> CFP\u306f\u901a\u5e38440 nm\u306e\u52b1\u8d77\u5149\u3067480 nm\u306e\u86cd\u5149\u3092\u767a\u3059\u308b\u3002\u3053\u3053\u306b\u30a8\u30cd\u30eb\u30ae\u30fc\u53d7\u5bb9\u4f53\u3067\u3042\u308bYFP\u304c\u8fd1\u63a5\u3057\u3066\u3044\u308b\u3068\u3001\u30a8\u30cd\u30eb\u30ae\u30fc\u9077\u79fb\u304c\u751f\u3058\u3001\u540c\u3058440 nm\u306e\u52b1\u8d77\u5149\u306b\u5bfe\u3057\u3066YFP\u306e\u86cd\u5149\uff08530 nm\uff09\u304c\u8a8d\u3081\u3089\u308c\u308b\u3002480\u3068530 nm\u306e\u86cd\u5149\u5f37\u5ea6\u6bd4\u3092\u6307\u6a19\u306bCFP\u3068YFP\u306e\u8ddd\u96e2\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/p>\n<h2>BiFC<\/h2>\n<p>EGFP\u306f240\u500b\u307b\u3069\u306e\u30a2\u30df\u30ce\u9178\u304b\u3089\u308a\u3001\u56f32\u306e\u5de6\u306e\u3088\u3046\u306a\u30d9\u30fc\u30bf\u30d0\u30ec\u30eb\u69cb\u9020\u3092\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u3092\u9069\u5f53\u306a\u4f4d\u7f6e\u3067\u5207\u65ad\u3059\u308b\u3068\u5404\u3005\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u5149\u3092\u767a\u3057\u307e\u305b\u3093\uff08\u56f3\uff12\u5de6\uff09\u3002\u3057\u304b\u3057\u4e21\u8005\u304c\u7d50\u5408\u3057\u305f\u6642\u306f\u5b8c\u5168\u306a\u69cb\u9020\u304c\u5f62\u6210\u3055\u308c\u86cd\u5149\u3092\u767a\u3057\u307e\u3059\uff08\u56f3\uff12\u53f3\uff09\u3002\u3053\u308c\u304c\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u518d\u69cb\u6210\u6cd5\uff08bimolecular fluorescence complementation, BiFC\uff09\u3067\u3059\u3002 \u76f8\u4e92\u4f5c\u7528\u3092\u8abf\u3079\u305f\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u305d\u308c\u305e\u308c\u3092\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u305d\u306e\u7d50\u5408\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u7d50\u5408\u306e\u5897\u52a0\u304c\u5358\u306b\u86cd\u5149\u5f37\u5ea6\u306e\u5897\u5f37\u3067\u691c\u51fa\u3067\u304d\u308b\u5358\u7d14\u3055\u304c\u3042\u308a\u3001GFP\u3092\u7528\u3044\u308c\u3070\u901a\u5e38\u306e\u86cd\u5149\u9855\u5fae\u93e1\u306b\u5099\u3048\u4ed8\u3051\u306eFITC\u30d5\u30a3\u30eb\u30bf\u30fc\u3060\u3051\u3067\u89b3\u5bdf\u53ef\u80fd\u3067\u3059\u3002\u307e\u305f\u3001\u76f8\u4e92\u4f5c\u7528\u304c\u5358\u8272\u3067\u691c\u51fa\u53ef\u80fd\u306a\u70b9\u306f\u9b45\u529b\u7684\u3067\u3001\u8907\u6570\u306e\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u306e\u540c\u6642\u89b3\u5bdf\u3082\u5bb9\u6613\u306b\u884c\u3048\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bifc.jpg\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-339\" src=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bifc.jpg\" alt=\"\" width=\"325\" height=\"193\" srcset=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bifc.jpg 325w, https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bifc-300x178.jpg 300w\" sizes=\"(max-width: 325px) 100vw, 325px\" \/><\/a><\/p>\n<p><strong>\u56f32 BiFC\u306b\u3088\u308b\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u306e\u691c\u51fa\u539f\u7406<\/strong> \u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaYFP\uff08Venus\uff09\u3092N\u672b\u3068C\u672b\u5074\u306b\u5206\u65ad\u3059\u308b\u3068\uff08\u56f3\u3067\u306f172-173\u30a2\u30df\u30ce\u9178\uff09\u3001\u86cd\u5149\u306f\u767a\u3057\u306a\u3044\u3002\u305d\u308c\u305e\u308c\u306b\u7d50\u5408\u304c\u671f\u5f85\u3055\u308c\u308b\u4e8c\u3064\u306e\u30bf\u30f3\u30d1\u30af\u8ceaA\u3068B\u3092\u878d\u5408\u3059\u308b\u3002A\u3068B\u306e\u7d50\u5408\u304cVenus\u306e\u69cb\u9020\u306e\u518d\u69cb\u6210\u3092\u8d77\u3053\u3057\u86cd\u5149\u3092\u767a\u3059\u308b\u305f\u3081\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u304c\u86cd\u5149\u5f37\u5ea6\u306e\u5897\u52a0\u3068\u3057\u3066\u89b3\u5bdf\u3055\u308c\u308b\u3002<\/p>\n<h2>\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9<\/h2>\n<p>\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306f\u7d30\u80de\u5916\u306e\u7269\u8cea\u3092\u7d30\u80de\u5185\u306b\u53d6\u308a\u8fbc\u3080\u4ed5\u7d44\u307f\u3067\u3059\u3002\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u819c\u306e\u69cb\u6210\u6210\u5206\u3084\u305d\u306e\u5f62\u6210\u6a5f\u69cb\u306b\u3088\u3063\u3066\u3001\u30af\u30e9\u30b9\u30ea\u30f3\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\uff08\u53d7\u5bb9\u4f53\u578b\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3068\u547c\u3070\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\uff09\u3001\u30ab\u30d9\u30aa\u30e9\u578b\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3001\u30af\u30e9\u30b9\u30ea\u30f3\u975e\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u7b49\u306b\u5206\u985e\u3055\u308c\u307e\u3059\u3002\u30af\u30e9\u30b9\u30ea\u30f3\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306f\u3001\u4e3b\u306b\u30ea\u30ac\u30f3\u30c9\u3068\u7d50\u5408\u3057\u305f\u53d7\u5bb9\u4f53\u304c\u7d30\u80de\u5185\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u305f\u3081\u306e\u6a5f\u69cb\u3067\u3059\u3002\u53d6\u308a\u8fbc\u307e\u308c\u305f\u5c0f\u80de\u306f\u30af\u30e9\u30b9\u30ea\u30f3\u304c\u8131\u88ab\u8986\u5316\u3057\u305f\u5f8c\u306b\u5c0f\u80de\u540c\u58eb\u304c\u878d\u5408\u3057\u3066\u521d\u671f\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u3068\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001\u4e00\u90e8\u306f\u5f8c\u671f\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u3001\u30ea\u30bd\u30bd\u30fc\u30e0\u3078\u3068\u9032\u3080\u5206\u89e3\u7d4c\u8def\u3001\u4e00\u90e8\u306f\u30ea\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u3078\u3068\u9032\u3080\u518d\u5229\u7528\u7d4c\u8def\u3078\u3068\u904b\u547d\u3092\u5206\u304b\u3061\u307e\u3059\u3002\u30ab\u30d9\u30aa\u30e9\u578b\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306f\u3001\u7d30\u80de\u819c\u5185\u8449\u306e\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u3068\u306e\u7d50\u5408\u3092\u4ecb\u3057\u3066\u81ea\u5df1\u4f1a\u5408\u3057\u305f\u30ab\u30d9\u30aa\u30ea\u30f3\u304c\u304f\u307c\u307f\u69cb\u9020\uff08\u30ab\u30d9\u30aa\u30e9\uff09\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u30af\u30e9\u30b9\u30ea\u30f3\u975e\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306f\u3001\u30af\u30e9\u30b9\u30ea\u30f3\u3084\u30ab\u30d9\u30aa\u30e9\u578b\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3068\u306f\u7570\u306a\u308a\u88ab\u8986\u5c0f\u80de\u3092\u5f62\u6210\u305b\u305a\u306b\u7269\u8cea\u3092\u53d6\u308a\u8fbc\u307f\u307e\u3059\u3002\u30de\u30af\u30ed\u30d4\u30ce\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3084\u30d5\u30a1\u30b4\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u7b49\u304c\u3053\u306e\u69d8\u5f0f\u306b\u542b\u307e\u308c\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h1>\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3068\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0<\/h1>\n<h2>Ras-PI3K\u30b7\u30b0\u30ca\u30eb\u306b\u3088\u308b\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u5236\u5fa1\u6a5f\u69cb<\/h2>\n<p>\u4f4e\u5206\u5b50\u91cfG\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308bRas\u306f\u3001\u4e0b\u6d41\u3067\u50cd\u304f\u8907\u6570\u306e\u6a19\u7684\u56e0\u5b50\u3092\u300c\u3044\u3064\u3001\u3069\u3053\u3067\u300d\u6d3b\u6027\u5316\u3055\u305b\u308b\u304b\u5de7\u5999\u306b\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u304a\u3044\u3066\u591a\u5f69\u306a\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002\u751f\u304d\u305f\u7d30\u80de\u306b\u304a\u3051\u308bRas\u3068\u305d\u306e\u6a19\u7684\u56e0\u5b50\u304c\u5f62\u6210\u3059\u308b\u8907\u5408\u4f53\u306e\u6319\u52d5\u3092BiFC\u6cd5\u3067\u53ef\u8996\u5316\u3057\u305f\u3068\u3053\u308d\u3001Ras\u3068phosphoinositide 3-kinase\uff08PI3K\uff09\u306e\u8907\u5408\u4f53\u306f\u3001\u4ed6\u306e\u6a19\u7684\u56e0\u5b50\u3068\u306f\u7570\u306a\u308a\u7d30\u80de\u819c\u3060\u3051\u3067\u306a\u304f\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u306b\u3082\u5c40\u5728\u3057\u3066\u3044\u307e\u3057\u305f\uff08\u56f31\uff09\u3002\u307e\u305f\u3001Ras-PI3K\u8907\u5408\u4f53\u304c\u7d30\u80de\u819c\u304b\u3089\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u306b\u79fb\u884c\u3057\u3001\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u304b\u3089\u767a\u4fe1\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u304c\u3001\u30af\u30e9\u30b9\u30ea\u30f3\u975e\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306e\u5236\u5fa1\u306b\u91cd\u8981\u306a\u3053\u3068\u3082\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\u3002 \u672c\u7814\u7a76\u306b\u3088\u308a\u3001\u3053\u308c\u307e\u3067\u7d30\u80de\u819c\u304c\u4e3b\u6226\u5834\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304d\u305fRas-PI3K\u30b7\u30b0\u30ca\u30eb\u306b\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u3068\u3044\u3046\u65b0\u305f\u306a\u6d3b\u8e8d\u306e\u5834\u3092\u898b\u51fa\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002\u3053\u306e\u767a\u898b\u306f\u3001\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306b\u5909\u5316\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u3092\u5b9f\u969b\u306b\u898b\u308b\u3053\u3068\u304b\u3089\u30b9\u30bf\u30fc\u30c8\u3057\u305f\u6210\u679c\u3067\u3042\u308a\u3001 \u201cseeing is believing\u201d\u3092\u307e\u3055\u306b\u4f53\u73fe\u3057\u305f\u597d\u4f8b\u3067\u3059\u3002<\/p>\n<p><a href=\"..\/wp-content\/uploads\/2014\/03\/ras_pi3k.jpg\"><img loading=\"lazy\" class=\"alignnone size-medium wp-image-346\" src=\"..\/wp-content\/uploads\/2014\/03\/ras_pi3k.jpg\" alt=\"ras_pi3k\" width=\"360\" height=\"120\" srcset=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/ras_pi3k.jpg 642w, https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/ras_pi3k-300x101.jpg 300w\" sizes=\"(max-width: 360px) 100vw, 360px\" \/><\/a><\/p>\n<p><strong>\u56f31\u3000Ras-PI3K\u8907\u5408\u4f53\u306f\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u306b\u5c40\u5728\u3059\u308b<\/strong> BiFC\u6cd5\u3092\u7528\u3044\u3066Ras\u3068\u6a19\u7684\u56e0\u5b50\u304c\u5f62\u6210\u3059\u308b\u8907\u5408\u4f53\u3092\u53ef\u8996\u5316\u3057\u305f\u3002Ras-PI3K\u8907\u5408\u4f53\u306f\u7d30\u80de\u819c\u3060\u3051\u3067\u306a\u304f\u3001\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u306b\u3082\u5c40\u5728\u3057\u305f\u3002<\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u3011<\/strong><\/p>\n<ul>\n<li>Visualization of Ras-PI3K interaction in the endosome using BiFC. Tsutsumi K, Fujioka Y, Tsuda M, Kawaguchi H, &amp; Ohba Y. <em><strong>Cell Signal.<\/strong><\/em> 21: 1672-1679, 2009<\/li>\n<\/ul>\n<h2>Ras-PI3K\u30b7\u30b0\u30ca\u30eb\u3068\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306e\u5bbf\u4e3b\u7d30\u80de\u53d6\u308a\u8fbc\u307f\u6a5f\u69cb<\/h2>\n<p>\u6e96\u5099\u4e2d<\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u3011<\/strong><\/p>\n<ul>\n<li>The Ras\u2013PI3K Signaling Pathway Is Involved in Clathrin-Independent Endocytosis and the Internalization of Influenza Viruses. Fujioka. Y, Tsuda M, Hattori T, Sasaki J, Sasaki T, Miyazaki T &amp; Ohba Y. <em><strong>PLoS ONE<\/strong><\/em> 6: e16324, 2011\n<ul>\n<li><a href=\"http:\/\/www.hokudai.ac.jp\/bureau\/topics\/press_release\/110121_pr_med_infle.pdf#search=\u2019\" target=\"_blank\" rel=\"noopener noreferrer\">\u5317\u5927\u306b\u3088\u308b\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\u8a18\u4e8b<\/a><\/li>\n<li><a href=\"http:\/\/www.natureasia.com\/ja-jp\/jobs\/tokushu\/detail\/227\" target=\"_blank\" rel=\"noopener noreferrer\">Nature Asia<\/a><\/li>\n<\/ul>\n<\/li>\n<li>A peptide derived from phosphoinositide 3-kinase inhibits endocytosis and influenza virus infection. Fujioka Y, Satoh A, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, &amp; Ohba Y. <em><strong>Cell Struct Funct<\/strong><\/em>\u00a044: 61-74, 2019\n<ul>\n<li><a href=\"http:\/\/www.jscb.gr.jp\/action\/thesis_winners.html\">\u65e5\u672c\u7d30\u80de\u751f\u7269\u5b66\u4f1a\u8ad6\u6587\u8cde<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h2>Ca<sup>2+<\/sup>\u30b7\u30b0\u30ca\u30eb\u3092\u4ecb\u3057\u305f\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306e\u5bbf\u4e3b\u7d30\u80de\u4fb5\u5165\u6a5f\u69cb<\/h2>\n<p>\u591a\u304f\u306e\u30a6\u30a4\u30eb\u30b9\u306f\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u969b\u306b\u7d30\u80de\u304c\u6301\u3064\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u6a5f\u69cb\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u306f\u30af\u30e9\u30b9\u30ea\u30f3\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3067\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u79c1\u305f\u3061\u306e\u7814\u7a76\u304b\u3089\u3001Ras-PI3K\u30b7\u30b0\u30ca\u30eb\u306e\u6d3b\u6027\u5316\u3092\u4ecb\u3057\u3066\u3001\u30af\u30e9\u30b9\u30ea\u30f3\u975e\u4f9d\u5b58\u6027\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3067\u3082\u4fb5\u5165\u53ef\u80fd\u306a\u3053\u3068\u304c\u8a3c\u660e\u3055\u308c\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u305d\u306e\u969b\u306b\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u306e\u4e0a\u6607\u3092\u5f15\u304d\u91d1\u306b\u3001Ras-PI3K\u30b7\u30b0\u30ca\u30eb\u3092\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\uff08\u56f3\uff12\uff09\u3002<\/p>\n<p><a href=\"..\/wp-content\/uploads\/2014\/03\/end01.jpg\"><img loading=\"lazy\" class=\"alignnone size-medium wp-image-342\" src=\"..\/wp-content\/uploads\/2014\/03\/end01.jpg\" alt=\"end01\" width=\"300\" height=\"65\" srcset=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/end01.jpg 600w, https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/end01-300x66.jpg 300w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><strong>\u56f32\u3000\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u306b\u3088\u308b\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6fc3\u5ea6\u306e\u4e00\u904e\u6027\u4e0a\u6607<\/strong> \u57f9\u990a\u7d30\u80de\u306b\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6fc3\u5ea6\u3092\u30e2\u30cb\u30bf\u30fc\u3059\u308bFRET\u30bb\u30f3\u30b5\u30fc\u3092\u767a\u73fe\u3055\u305b\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u52d5\u614b\u3092\u751f\u304d\u305f\u7d30\u80de\u3067\u89b3\u5bdf\u3057\u305f\u3002\u611f\u67d3\u76f4\u5f8c\u306b\u3001\u8907\u6570\u56de\u306e\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6fc3\u5ea6\u306e\u4e00\u904e\u7684\u4e0a\u6607\u304c\u8d77\u3053\u3063\u305f\u3002<\/p>\n<p>\u3055\u3089\u306b\u89e3\u6790\u3092\u9032\u3081\u308b\u3068\u3001\u30a6\u30a4\u30eb\u30b9\u304c\u611f\u67d3\u3057\u305f\u6642\u306b\u751f\u3058\u308b\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u4e0a\u6607\u3092\u304d\u3063\u304b\u3051\u306b\u3001Ras-PI3K\u30b7\u30b0\u30ca\u30eb\u306e\u307f\u306a\u3089\u305a\u69d8\u3005\u306a\u30b7\u30b0\u30ca\u30eb\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u304c\u767a\u52d5\u3057\u3001\u7a2e\u3005\u306e\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u304c\u4fc3\u9032\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\uff08\u56f3\uff13\uff09\u3002 \u3059\u306a\u308f\u3061\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306f\u5bbf\u4e3b\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u306a\u3069\u3001\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u6a5f\u69cb\u3092\u4e57\u3063\u53d6\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u5185\u306b\u4fb5\u5165\u3057\u3084\u3059\u3044\u74b0\u5883\u3092\u4f5c\u308a\u51fa\u3057\u3066\u3044\u308b\u3068\u3044\u3046\u30e2\u30c7\u30eb\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u672c\u7814\u7a76\u306e\u3088\u3046\u306b\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u7528\u3044\u3066\u7d30\u80de\u306e\u6a5f\u80fd\u3092\u63a2\u6c42\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u3053\u308c\u307e\u3067\u306f\u660e\u3089\u304b\u3067\u306f\u306a\u304b\u3063\u305f\u7d30\u80de\u306b\u6f5c\u3080\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u89e3\u660e\u304c\u52a0\u901f\u3059\u308b\u3082\u306e\u3068\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n<p><a href=\"..\/wp-content\/uploads\/2014\/03\/end02.jpg\"><img loading=\"lazy\" class=\"alignnone size-medium wp-image-343\" src=\"..\/wp-content\/uploads\/2014\/03\/end02.jpg\" alt=\"end02\" width=\"300\" height=\"160\" srcset=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/end02.jpg 489w, https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/end02-300x160.jpg 300w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><strong>\u56f33\u3000\u30ab\u30eb\u30b7\u30a6\u30e0\u30b7\u30b0\u30ca\u30eb\u3092\u4ecb\u3057\u305f\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306e\u4fb5\u5165\u6a5f\u69cb<\/strong> \u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306f\u7d30\u80de\u306b\u5438\u7740\u3059\u308b\u3068\u3059\u3050\u3055\u307e\u3001\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u4e0a\u6607\u2192RhoA\u6d3b\u6027\u5316\u2192\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u4e0a\u6607\u3068\u3044\u3046\u30b7\u30b0\u30ca\u30eb\u3092\u767a\u52d5\u3055\u305b\u3001\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3092\u4ea2\u9032\u3059\u308b\u3053\u3068\u3067\u52b9\u7387\u7684\u306b\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u3002<\/p>\n<p>\u3055\u3089\u306b\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u304c\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u4e0a\u6607\u3055\u305b\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u8abf\u3079\u305f\u3068\u3053\u308d\u3001\u96fb\u4f4d\u4f9d\u5b58\u6027\u30ab\u30eb\u30b7\u30a6\u30e0\u30c1\u30e3\u30cd\u30eb\u304c\u9375\u3067\u3042\u308b\u3053\u3068\u304c\u89e3\u308a\u307e\u3057\u305f\u3002\u96fb\u4f4d\u4f9d\u5b58\u6027\u30ab\u30eb\u30b7\u30a6\u30e0\u30c1\u30e3\u30cd\u30eb\u306e\u963b\u5bb3\u85ac\u3067\u3042\u308b\u30ab\u30eb\u30b7\u30a6\u30e0\u30d6\u30ed\u30c3\u30ab\u30fc\u306fin vitro\u3060\u3051\u3067\u306a\u304f\u30de\u30a6\u30b9\u3092\u7528\u3044\u305fin vivo\u3067\u306e\u7cfb\u3084\u30d2\u30c8\u7d30\u80de\u3092\u7528\u3044\u305fex vivo\u306e\u5b9f\u9a13\u7cfb\u3067\u3082\u611f\u67d3\u6291\u5236\u52b9\u679c\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u4eca\u5f8c\u306e\u6297\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u85ac\u958b\u767a\u3078\u306e\u5c55\u958b\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u3011<\/strong><\/p>\n<ul>\n<li>A Ca<sup>2+<\/sup>-dependent signalling circuit regulates influenza A virus internalisation and infection. Fujioka Y, Tsuda M, Nanbo A, Hattori T, Sasaki J, Sasaki T, Miyazaki T &amp; Ohba Y.<em><strong> Nat. Commun.<\/strong><\/em> 4: 2763, 2013\n<ul>\n<li><a href=\"http:\/\/www.hokudai.ac.jp\/news\/131114_pr_med.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">\u5317\u5927\u306b\u3088\u308b\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\u8a18\u4e8b<\/a><\/li>\n<li>Yahoo\u30cb\u30e5\u30fc\u30b9<\/li>\n<li>\u5317\u6d77\u9053\u306e\u30cb\u30e5\u30fc\u30b9\u30fb\u6c17\u8c61\u60c5\u5831\u3001\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30cb\u30e5\u30fc\u30b9\u5317\u6d77\u9053\uff08\u3068\u3082\u306bNHK\u300111\/14\uff09<\/li>\n<\/ul>\n<\/li>\n<li>A Sialylated Voltage-Dependent Ca<sup>2+<\/sup> Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells. Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh A, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y. <em><strong>Cell Host Microbe<\/strong><\/em> 23: 809-818, 2018\n<ul>\n<li><a href=\"https:\/\/www.cell.com\/cell-host-microbe\/fulltext\/S1931-3128(18)30273-7\" target=\"_blank\" rel=\"noopener noreferrer\">Previews<\/a><\/li>\n<li><a href=\"https:\/\/www.hokudai.ac.jp\/news\/180523_pr.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"> \u5317\u5927\u306b\u3088\u308b\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\u8a18\u4e8b<\/a><\/li>\n<li><a href=\"http:\/\/first.lifesciencedb.jp\/archives\/18288\" target=\"_blank\" rel=\"noopener noreferrer\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u65b0\u7740\u8ad6\u6587\u30ec\u30d3\u30e5\u30fc\u00a0<\/a><\/li>\n<li><a href=\"https:\/\/news.mynavi.jp\/article\/20180529-637561\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u30de\u30a4\u30ca\u30d3\u30cb\u30e5\u30fc\u30b9<\/a><\/li>\n<li><a href=\"https:\/\/yomidr.yomiuri.co.jp\/article\/20180607-OYTET50009\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u8aad\u58f2\u65b0\u805e<\/a><\/li>\n<li><a href=\"https:\/\/www.nikkan.co.jp\/articles\/view\/00475170\" target=\"_blank\" rel=\"noopener noreferrer\">\u65e5\u520a\u5de5\u696d\u65b0\u805e<\/a><\/li>\n<li><a href=\"http:\/\/www.microbe.tv\/twiv\/twiv-495\/\" target=\"_blank\" rel=\"noopener noreferrer\">This week in Virology<\/a><\/li>\n<li><a href=\"https:\/\/issuu.com\/asiaresearchnews\/docs\/arn-2019_online_magazine?e=1764199\/67236192\" target=\"_blank\" rel=\"noopener noreferrer\">Asia Research News<\/a> \u4ed6<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h1>\u30aa\u30eb\u30ac\u30cd\u30e9\u30c0\u30a4\u30ca\u30df\u30af\u30b9<\/h1>\n<h2>\u30aa\u30eb\u30ac\u30cd\u30e9\u30de\u30fc\u30ab\u30fc\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u69cb\u7bc9<\/h2>\n<p>\u69d8\u3005\u306a\u30aa\u30eb\u30ac\u30cd\u30e9\u3092\u751f\u304d\u305f\u7d30\u80de\u306b\u304a\u3044\u3066\u53ef\u8996\u5316\u3059\u308b\u305f\u3081\u306e\u30de\u30fc\u30ab\u30fc\u3092\u4f5c\u88fd\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u73fe\u5728\u4f5c\u88fd\u6e08\u307f\u306e\u3082\u306e\u3068\u3057\u3066\u306f\u3001\u6838\u3001\u7d30\u80de\u819c\u3001\u30a2\u30af\u30c1\u30f3\u3001\u5fae\u5c0f\u7ba1\u3001\u63a5\u7740\u6591\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3001\u521d\u671f\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u3001\u5f8c\u671f\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u3001\u30ea\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u30a8\u30f3\u30c9\u30be\u30fc\u30e0\u3001\u30b4\u30eb\u30b8\u88c5\u7f6e\u3001\u5c0f\u80de\u4f53\u3067\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u30aa\u30eb\u30ac\u30cd\u30e9\u306b\u5bfe\u3057\u3066\u3001Sirius\uff08\u7fa4\u9752\uff09\u3001SECFP\uff08\u30b7\u30a2\u30f3\uff09\u3001EGFP\uff08\u7dd1\uff09\u3001Venus\uff08\u9ec4\uff09\u3001tdTomato\uff08\u8d64\uff09\u3001mCherry\uff08\u8d64\uff09\u3001TFP650\uff08\u8fd1\u8d64\u5916\uff09\u3001iRFP713\uff08\u8fd1\u8d64\u5916\uff09\u3001Keima\uff08\u9752\u8272\u52b1\u8d77\u3067\u8d64\u8272\u86cd\u5149\uff09\u7b49\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u878d\u5408\u3057\u305f\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u3092\u4f5c\u88fd\u3057\u3001\u30de\u30fc\u30ab\u30fc\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u69cb\u7bc9\u3057\u3066\u304a\u308a\u307e\u3059\u3002 \u3053\u306e\u904e\u7a0b\u3067\u79c1\u9054\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u30de\u30fc\u30ab\u30fc\u3084\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u30de\u30fc\u30ab\u30fc\u306b\u3001\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u65e9\u3044\uff08\u3059\u306a\u308f\u3061\u300c\u660e\u308b\u3044\u300d\uff09\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u878d\u5408\u3055\u305b\u308b\u3068\u3001\u672c\u6765\u306e\u5c40\u5728\u5316\u304c\u963b\u5bb3\u3055\u308c\u3001\u7d30\u80de\u8cea\u3084\u6838\u306b\u6f0f\u308c\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002<\/p>\n<p style=\"padding-left: 30px;\">\u30b5\u30f3\u30d7\u30eb\u30ea\u30af\u30a8\u30b9\u30c8\u306f<a href=\".\/research\/requests.html\">\u3053\u3061\u3089<\/a><\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u3011<\/strong><\/p>\n<ul>\n<li>Folding latency of fluorescent proteins affects the mitochondrial localization of fusion proteins. Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, Tsuzuki A, Aoki O, Paudel S, Sasajima H &amp; <u>Ohba Y<\/u>. <strong><em>Cell Struct. Funct.\u00a0<\/em><\/strong>44: 183-194, 2019<\/li>\n<li>Localization of BCR-ABL to stress granules contributes to its oncogenic function. Kashiwagi S, Fujioka Y, Kondo T, Satoh AO, Yoshida A, Fujioka M, Sasajima H, Amano M, Teshima T &amp; Ohba Y.\u00a0<strong><em>Cell Struct. Funct.<\/em><\/strong> 44: 195-204, 2019<\/li>\n<li>Attenuation of ligand-induced activation of angiotensin II type 1 receptor signaling by the type 2 receptor via protein kinase C. Inuzuka T, Fujioka Y, Tsuda M, Fujioka M, Satoh AO, Horiuchi K, Nishide S, Nanbo A, Tanaka S &amp; Ohba Y. <strong><em>Sci. Rep.<\/em><\/strong> 6: 21613, 2016<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p id=\"pickles\">\n<h1>FRET\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306e\u81e8\u5e8a\u5fdc\u7528<\/h1>\n<h2>\u301c\u5149\u8a3a\u65ad\u85ac\u306e\u958b\u767a\u3068\u4e8b\u696d\u5316\u301c<\/h2>\n<p>\u86cd\u5149\u30d0\u30a4\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306f\u3001\u751f\u304d\u305f\u7d30\u80de\u5185\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u5909\u5316\u3092\u3001\u9ad8\u611f\u5ea6\u304b\u3064\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u6280\u8853\u3067\u3059\u3002\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u3053\u306e\u7279\u9577\u3092\u6d3b\u304b\u3057\u3066\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3092\u81e8\u5e8a\u691c\u67fb\u306b\u5fdc\u7528\u3059\u308b\u3053\u3068\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u6162\u6027\u9aa8\u9ac4\u6027\u767d\u8840\u75c5\uff08chronic myeloid leukemia, CML\uff09\u306f\u3001\u9aa8\u9ac4\u306e\u9020\u8840\u5e79\u7d30\u80de\u306b\u7570\u5e38\u67d3\u8272\u4f53\uff08\u30d5\u30a3\u30e9\u30c7\u30eb\u30d5\u30a3\u30a2\u67d3\u8272\u4f53, Ph1\uff09\u304c\u5f62\u6210\u3055\u308c\u3001\u767d\u8840\u75c5\u306e\u539f\u56e0\u30bf\u30f3\u30d1\u30af\u8ceaBCR-ABL\u304c\u7d30\u80de\u5185\u306b\u51fa\u73fe\u3057\u767a\u75c7\u3059\u308b\u8840\u6db2\u306e\u304c\u3093\u3067\u3059\u3002BCR\uff70ABL\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u306b\u5bfe\u3059\u308b\uff13\u7a2e\u985e\u306e\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u304c\u6a19\u6e96\u6cbb\u7642\u306b\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002 \u3057\u304b\u3057\u3001\u60a3\u8005\u3055\u3093\u3054\u3068\u306b\u3069\u306e\u85ac\u304c\u6709\u52b9\u306a\u306e\u304b\u3001\u5b9f\u969b\u306b\u6cbb\u7642\u3092\u59cb\u3081\u3066\u307f\u308b\u307e\u3067\u306f\u5206\u304b\u3089\u306a\u3044\u3053\u3068\u304c\u8ab2\u984c\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u6cbb\u7642\u524d\u304b\u3089\u500b\u3005\u306e\u60a3\u8005\u3055\u3093\u306b\u5bfe\u3057\u3066\u85ac\u306e\u6709\u52b9\u6027\u3092\u5224\u5b9a\u3057\u305f\u308a\u3001\u6cbb\u7642\u4e2d\u306b\u85ac\u5264\u8010\u6027\u7d30\u80de\u306e\u6709\u7121\u3092\u691c\u51fa\u3057\u305f\u308a\u3059\u308b\u6280\u8853\u304c\u3042\u308c\u3070\u3001\u306f\u3058\u3081\u304b\u3089\u52b9\u679c\u7684\u306a\u6cbb\u7642\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bio_censor01.jpg\"><img loading=\"lazy\" src=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bio_censor01-150x150.jpg\" alt=\"\" width=\"150\" height=\"150\" class=\"alignnone size-thumbnail wp-image-340\" \/><\/a><\/p>\n<p><strong>\u56f31\u3000BCR-ABL\u6d3b\u6027\u6e2c\u5b9a\u30bb\u30f3\u30b5\u30fcPickles<\/strong> BCR-ABL\u306e\u57fa\u8cea\u3067\u3042\u308bCrkL\u306f\u30ea\u30f3\u9178\u5316\u3055\u308c\u308b\u3068\u69cb\u9020\u5909\u5316\u3059\u308b\u3002CrkL\u306e\u4e21\u7aef\u306bYFP\uff08Yellow fluorescent protein, \u9ec4\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3068CFP\uff08Cyan fluorescent protein, \u9752\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3092\u4ed8\u3051\u308b\u3053\u3068\u3067\u3001BCR-ABL\u306e\u6d3b\u6027\u306b\u5fdc\u7b54\u3057\u3066FRET\u304c\u751f\u3058\u308b\u30bb\u30f3\u30b5\u30fc\u306e\u958b\u767a\u306b\u6210\u529f\u3057\u305f\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306fBCR-ABL\u306e\u57fa\u8ceaCrkL\u3068FRET\u306e\u539f\u7406\u3092\u5229\u7528\u3057\u305f\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u3000Pickles\uff08Phosphorylation indicator of CrkL en substrate\u3001\u56f31\uff09\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u305d\u308c\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u5b9f\u969b\u306b\u60a3\u8005\u3055\u3093\u304b\u3089\u63a1\u53d6\u3057\u305fCML\u7d30\u80de\u304c\u3069\u306e\u3088\u3046\u306a\u85ac\u5264\u53cd\u5fdc\u6027\u3092\u793a\u3059\u304b\u3092\u4e00\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u89e3\u6790\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3057\u307e\u3057\u305f\uff08\u56f32\uff09\u3002<\/p>\n<p><a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bio_censor02.jpg\"><img loading=\"lazy\" src=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-content\/uploads\/2014\/03\/bio_censor02-150x150.jpg\" alt=\"\" width=\"150\" height=\"150\" class=\"alignnone size-thumbnail wp-image-341\" \/><\/a><\/p>\n<p><strong>\u56f32\u3000Pickles\u306b\u3088\u308b\u85ac\u52b9\u8a55\u4fa1\u30d7\u30ed\u30bb\u30b9<\/strong> \u60a3\u8005\u3055\u307e\u304b\u3089\u5f97\u305f\u8840\u7403\u304b\u3089\u3001\u5358\u6838\u7403\u5206\u753b\u3092\u5358\u96e2\u3057\u3001Pickles\u3092\u5c0e\u5165\u3059\u308b\u300224\u6642\u9593\u5f8c\u306b\u306f\u30bb\u30f3\u30b5\u30fc\u5206\u5b50\u306e\u86cd\u5149\u304c\u89b3\u5bdf\u53ef\u80fd\u3067\u3042\u308a\uff0c\u9855\u5fae\u93e1\u3067\u4e00\u3064\u4e00\u3064\u306eFRET\u52b9\u7387\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3002\u85ac\u5264\u51e6\u7406\u5f8c\u306b\u3059\u3079\u3066\u306e\u7d30\u80de\u3067BCR-ABL\u306e\u6d3b\u6027\u304c\u6291\u5236\uff08FRET\u4f4e\uff09\u3055\u308c\u3066\u3044\u308c\u3070\u85ac\u5264\u304c\u6709\u52b9\u3001\u62b5\u6297\u6027\u306e\u5834\u5408\u306b\u306fFRET\u306e\u9ad8\u3044\u7d30\u80de\u304c\u6b8b\u5b58\u3059\u308b\u3002<\/p>\n<p>\u672c\u6280\u8853\u3092\u6cbb\u7642\u524d\u306eCML\u60a3\u8005\u3055\u3093\u306b\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u6709\u52b9\u306a\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u3092\u5b9f\u969b\u306b\u4e88\u6e2c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u500b\u3005\u306e\u60a3\u8005\u3055\u3093\u306b\u30d5\u30a3\u30c3\u30c8\u3057\u305f\u6cbb\u7642\u85ac\u3092\u3042\u3089\u304b\u3058\u3081\u9078\u629e\u3067\u304d\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u672c\u624b\u6cd5\u306b\u306f\u300c\u7d30\u80de\u3092\u751f\u304d\u305f\u307e\u307e\u89e3\u6790\u3059\u308b\u300d\u3068\u3044\u3046\u3001\u3053\u308c\u307e\u3067\u306e\u691c\u67fb\u306b\u306f\u306a\u3044\u7279\u9577\u304c\u3042\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u85ac\u5264\u8010\u6027\u7d30\u80de\u306e\u307f\u3092\u96c6\u3081\u308b\u3053\u3068\u3082\u53ef\u80fd\u306a\u3053\u3068\u304b\u3089\u3001\u85ac\u5264\u8010\u6027\u7372\u5f97\u6a5f\u69cb\u306e\u89e3\u660e\u3078\u767a\u5c55\u3055\u305b\u308b\u3053\u3068\u3082\u671f\u5f85\u3067\u304d\u307e\u3059\u3002 \u3055\u3089\u306b\u3001\u4ed6\u306e\u30ad\u30ca\u30fc\u30bc\u6d3b\u6027\u3092\u6e2c\u5b9a\u3059\u308b\u5149\u8a3a\u65ad\u85ac\u3092\u958b\u767a\u3059\u308b\u3053\u3068\u3067\u3001\u7406\u8ad6\u7684\u306b\u306f\u5206\u5b50\u6a19\u7684\u6cbb\u7642\u85ac\u3092\u7528\u3044\u308b\u3059\u3079\u3066\u306e\u304c\u3093\u306b\u5fdc\u7528\u62e1\u5927\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u672c\u7814\u7a76\u306f\u3001\u79d1\u5b66\u6280\u8853\u632f\u8208\u6a5f\u69cb\uff08JST\uff09\u306e\u300c\u793e\u4f1a\u9084\u5143\u52a0\u901f\u30d7\u30ed\u30b0\u30e9\u30e0\uff08SCORE\uff09\u300d\uff082019\u5e74\u5ea6\uff09\u3001\u30c6\u30eb\u30e2\u751f\u547d\u79d1\u5b66\u632f\u8208\u8ca1\u56e3\u306e\u300c\u7814\u7a76\u958b\u767a\u52a9\u6210\uff08\u958b\u767a\u52a9\u6210\uff09\u300d\u3001\u56fd\u7acb\u7814\u7a76\u958b\u767a\u6cd5\u4eba\u65b0\u30a8\u30cd\u30eb\u30ae\u30fc\u30fb\u7523\u696d\u6280\u8853\u7dcf\u5408\u958b\u767a\u6a5f\u69cb\uff08NEDO\uff09\u306e\u300c\u7814\u7a76\u958b\u767a\u578b\u30b9\u30bf\u30fc\u30c8\u30a2\u30c3\u30d7\u652f\u63f4\u4e8b\u696d\/NEDO Entrepreneurs Program(NEP)\u30bf\u30a4\u30d7B\u300d\uff082021\u5e74\u5ea6\uff09\u3001\u300c\u7814\u7a76\u958b\u767a\u578b\u30b9\u30bf\u30fc\u30c8\u30a2\u30c3\u30d7\u652f\u63f4\u4e8b\u696d\uff0f\u30b7\u30fc\u30c9\u671f\u306e\u7814\u7a76\u958b\u767a\u578b\u30b9\u30bf\u30fc\u30c8\u30a2\u30c3\u30d7\u306b\u5bfe\u3059\u308b\u4e8b\u696d\u5316\u652f\u63f4(STS)\u300d\uff082022\u5e74\u5ea6\uff09\u306e\u3054\u652f\u63f4\u3092\u3044\u305f\u3060\u304d\u3001\u4e00\u65e5\u3082\u65e9\u304f\u60a3\u8005\u3055\u307e\u306e\u624b\u5143\u306b\u672c\u6280\u8853\u304c\u5c4a\u304f\u3088\u3046\u306b\u3001\u4e8b\u696d\u5316\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002 <\/p>\n<p>\u8a73\u3057\u304f\u306f<a href=\"https:\/\/www.horizonillumination.co.jp\/\">HILO\u682a\u5f0f\u4f1a\u793e<\/a>\u306e\u30da\u30fc\u30b8\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><strong>\u3010\u95a2\u9023\u696d\u7e3e\u30fb\u53c2\u8003\u6587\u732e\u3011<\/strong><\/p>\n<ul>\n<li><span style=\"font-size: inherit;\">A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, &amp; <\/span><strong style=\"font-size: inherit;\"><u>Ohba Y<\/u><\/strong><span style=\"font-size: inherit;\">*. <\/span><em style=\"font-size: inherit;\"><strong>Clin. Cancer\u00a0Res.<\/strong><\/em><span style=\"font-size: inherit;\"> 16(15): 3964-3975, 2010<\/span>\n<ul>\n<li>Commentary on this paper: <em><strong>Clin. Cancer Res.<\/strong><\/em> 16(15): 3822-3824 (2010)<\/li>\n<li><a href=\"http:\/\/aacrnews.wordpress.com\/2010\/07\/29\/new-lab-test-could-identify-imatinib-resistance\/\" target=\"_blank\" rel=\"noopener noreferrer\">AACR\uff08\u7c73\u56fd\u304c\u3093\u5b66\u4f1a\uff09<\/a><\/li>\n<li><a href=\"http:\/\/www.hokudai.ac.jp\/bureau\/topics\/press_release\/100729_pr_med.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">\u5317\u6d77\u9053\u5927\u5b66<\/a><\/li>\n<li>\u65e5\u7d4c\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\uff08\u30ea\u30f3\u30af\u5207\u308c\uff09<\/li>\n<li>\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30cb\u30e5\u30fc\u30b9\u5317\u6d77\u9053\uff08NHK\u30017\/29)<\/li>\n<li>\u9053\u65b0\u30cb\u30e5\u30fc\u30b9\uff08\u30c6\u30ec\u30d3\u5317\u6d77\u9053\u30017\/31\uff09<\/li>\n<li>\u5317\u6d77\u9053\u65b0\u805e\uff087\/30\u671d\u520a\uff09<\/li>\n<li>\u8aad\u58f2\u65b0\u805e\uff087\/29\u671d\u520a\uff09<\/li>\n<li>\u6bce\u65e5\u65b0\u805e\uff087\/30\u671d\u520a\uff09<\/li>\n<li>\u30ed\u30a4\u30bf\u30fc\u901a\u4fe1<\/li>\n<li>BTJ \u30a2\u30ab\u30c7\u30df\u30c3\u30af 7\/29\u306e\u30a2\u30af\u30bb\u30b9\u30e9\u30f3\u30ad\u30f3\u30b0\uff11\u4f4d\uff01<\/li>\n<\/ul>\n<\/li>\n<li><span style=\"font-size: inherit;\">Improved FRET biosensor for the measurement of BCR-ABL activity in chronic myeloid leukemia cells. Horiguchi M, Fujioka M, Kondo T, Fujioka Y, Li X, Horiuchi K, Satoh A, Nepal P, Nishide S, Nanbo A, Teshima T, and <\/span><strong style=\"font-size: inherit;\"><u>Ohba Y<\/u><\/strong><span style=\"font-size: inherit;\">*.\u00a0<\/span><em style=\"font-size: inherit;\"><strong>Cell Struct Funct<\/strong><\/em><span style=\"font-size: inherit;\"> 42, 15-26, 2017<\/span><\/li>\n<li><span style=\"font-size: inherit;\">Pre-treatment evaluation of FRET-based drug sensitivity test for patients with CML treated with dasatinib. Kondo T, et al, <\/span><strong style=\"font-size: inherit;\"><em>Cancer Sci. <\/em><\/strong><span style=\"font-size: inherit;\">109(7): 2256-2265, 2018<\/span><\/li>\n<li><span style=\"font-size: inherit;\">Clinical efficacy and safety of first-line nilotinib therapy and evaluation of the clinical utility of the FRET-based drug sensitivity test. Kondo T, et al. <\/span><strong style=\"font-size: inherit;\"><em>Int. J. Hematol.<\/em><\/strong><span style=\"font-size: inherit;\"> 110(4): 482-489, 2019<\/span><\/li>\n<li><span style=\"font-size: inherit;\">\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7814\u7a762008-&#8217;09 \u2015\u75be\u60a3\u767a\u75c7\u306e\u5206\u5b50\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u5b9f\u73fe\u5316\u3059\u308b\u5206\u5b50\u6a19\u7684\u85ac\u958b\u767a\u2015\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u53ef\u8996\u5316\u3068\u305d\u306e\u5275\u85ac\u3078\u306e\u5fdc\u7528 \u2002 <\/span><strong style=\"font-size: inherit;\"><u>\u5927\u5834\u96c4\u4ecb<\/u><\/strong><span style=\"font-size: inherit;\">\u3001\u6d25\u7530\u771f\u5bff\u7f8e \u2002 <\/span><strong style=\"font-size: inherit;\">\u5b9f\u9a13\u533b\u5b66<\/strong><span style=\"font-size: inherit;\"> 25(15)\uff08\u5897\u520a\uff09\uff1a 206-213 (2506-2513), 2008<\/span><\/li>\n<li><span style=\"font-size: inherit;\">\u30d0\u30a4\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306e\u57fa\u790e\u3068\u5fdc\u7528 \u2002 \u6d25\u7530\u771f\u5bff\u7f8e\u3001<\/span><strong style=\"font-size: inherit;\"> <u>\u5927\u5834\u96c4\u4ecb<\/u><\/strong><span style=\"font-size: inherit;\"> \u2002 <\/span><strong style=\"font-size: inherit;\">\u7d30\u80de<\/strong><span style=\"font-size: inherit;\"> 41(11): 462-463, 2009<\/span><\/li>\n<li>Localization of BCR-ABL to stress granules contributes to its oncogenic function. Kashiwagi S, Fujioka Y, Kondo T, Satoh AO, Yoshida A, Fujioka M, Sasajima H, Amano M, Teshima T &amp; Ohba Y.\u00a0<strong><em>Cell Struct. Funct.<\/em><\/strong> 44: 195-204, 2019<\/li>\n<\/ul>\n<p id=\"publication\">\n<p>&nbsp;<\/p>\n<h1><a href=\"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/publication.html\">\u7814\u7a76\u696d\u7e3e<\/a><\/h1>\n","protected":false},"excerpt":{"rendered":"<p>\u30d3\u30b8\u30e7\u30f3 \u751f\u547d\uff08\u3044\u306e\u3061\uff09\u306e\u7406\uff08\u3053\u3068\u308f\u308a\uff09 &hellip; <\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/pages\/6983"}],"collection":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/comments?post=6983"}],"version-history":[{"count":18,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/pages\/6983\/revisions"}],"predecessor-version":[{"id":7089,"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/pages\/6983\/revisions\/7089"}],"wp:attachment":[{"href":"https:\/\/c-mng.cwh.hokudai.ac.jp\/cp.med\/Root\/wp-json\/wp\/v2\/media?parent=6983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}